-- Creative Proteomics has launched a high-resolution analytical platform that allows the concurrent profiling of both water-soluble and fat-soluble vitamins with extraordinary sensitivity, eliminating major technical hurdles in metabolomics research.
Using the combined capabilities of pentafluorophenyl (PFP) chromatographic separation and polarity-switching UHPLC-MS/MS technology, it achieves attomole detection limits for all vitamins in the vitamin metabolome, which is critically relevant for researchers examining vitamin metabolism in precious biospecimens.
Researchers who have utilized the platform said the ability to characterize vitamin microheterogeneous states in a single analytical run represented a step forward compared to traditional workflows where hydrophilic and lipophilic vitamins would be assessed in separate workflows.
“The technical limitations of vitamin analysis have left researchers with a discovery challenge in metabolomics,” said Creative Proteomics’ principal investigator, who uses the platform in her own studies. “Getting the complete picture of the vitamin metabolome, including all interconversion states and metabolites, adds meaningful context to the disruption of other metabolic pathways.”
The analytical platform was developed to overcome certain barriers that the vitamin industry has struggled with for years, including biochemists' inability to differentiate between substituted metabolites—the biologically active vitamers and their inactive counterparts. This platform can resolve 24 different B6 vitamer states, and discriminate between vitamin D epimers that have considerably different biological activity—something that is more important in clinical research and pharmaceutical development.
Since the service specifications indicate superb reproducibility (CV <5%) with a dynamic range across 5 orders of magnitude that can span everything from cell culture models to clinical samples, the methods were specifically designed to be accurate regardless of the biological matrix being analyzed with the benefit of isotopically-labeled internal standards to ensure robust analyte quantitation.
“Vitamin metabolomics is not simply about understanding vitamin nutrient status, these compounds serve as cofactors to hundreds of enzymes with physiological roles. Knowing about availability and turnover of these compounds contributes to better understanding mechanisms related to pathophysiological conditions ranging from cancer metabolism to neurodegeneration,” said Creative Proteomics’ Director of Metabolomics.
Recent papers published in Nature Metabolism and Cell Metabolism highlighted that erroneous conclusions were reached in some disease models due to incomplete vitamin profiling. This new service provides researchers with in-depth vitamin metabolome characterization and incorporates bioinformatics analysis to link vitamins with the enzymatic demands.
This service will be of particular interest to pharmaceutical researchers developing drugs in the early phase of clinical trials as we know the drug-nutrient interaction plays a significant role in determining therapeutic response. Multiple large pharmaceutical companies have already integrated this platform into their drug development pipelines.
Scientists at Creative Proteomics reported that they conducted and validated this methodology for months across diverse sample types (plasma, tissue extracts, cell lysates, etc. and other matrices including CSF). The vitamin metabolomics service is ready for all academic and commercial-scale researchers, theirs for orders globally. Technical specifications, details about sample submissions, applications notes and more can be found on Creative Proteomics' website.
About Creative Proteomics
Creative Proteomics is a leading provider of proteomics, metabolomics, and bioinformatics services. With a focus on innovation and quality, the company supports researchers worldwide in advancing their scientific endeavors.
Contact Info:
Name: Melissa George
Email: Send Email
Organization: Creative Proteomics
Website: https://www.creative-proteomics.com/
Release ID: 89161802
Google
RSS